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Experimental study about dexamethasone to the proliferation of Rat aortic smooth muscle cells
王建波1，杨建勇1，陈伟1，庄文权1，张泷娟2
Jianbo Wang1，Jianyong Yang1，Wei Chen1，Wenquan Zhuang1，Longjuan Zhang2
（1.Department of Interventional Radiology，2.Laboratory of Surgery，The First Affiliated Hospital，SUN Yat－sen University，Guangzhou 510080，China）
（中山大学第一附属医院 1 放射介入科；2 外科实验室 510080）
Abstract
Objective：To evaluate the effect of dexamethasone to the cultured Rat thoracic aortic smooth muscle cells（SMC） in vitro.
Method：The Rat thoracic aortic SMC were harvest and cultured for six to ten passages. Cultured SMC were synchronized，then restimulated to enter the cell cycle，and treated with incremental concentrations of dexamethasone or without dexamethasone as contrast. The proliferative assay was performed with MTT method in the different time points after treated. DNA synthesis was determined 48 hours after restimulation by measuring [3H] thymidine incorporation. RT－PCR was performed to assay the level of proliferating cell nuclear antigen （PCNA） mRNA. | 
