Experimental Study on Preparation and Characteristics of Lipid Microbubbles loading VEGF Gene<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" />
李兴升 王志刚 杨春江 张群霞 冉海涛 景香香李攀
Li Xingsheng，Wang Zhigang，Yang Chunjiang，etal.
Institute of ultrasound imaging，Chongqing university of medical sciences，Chongqing 400010，China
Abstract
Objective：To prepare a kind of self—made lipid microbubble，to study its characteristics and the gene loading ability in vitro and to assess its enhancement effects in contrast imaging on normal rabbit kidneys .
Methods：A kind of lipid microbubble was prepared，and its appearance，concentration，mean diameter and potential were detected before and after irradiance of 60Co γ Ray，and the gene loading ability of the microbubble were detected. Seven normal rabbits were examined to observe the enhancement time and intensity in kidney parenchyma.
Results：The mean diameter range of the self—made lipid microbubble was 2.11—6.43um，the mean diameter was 2.79um and the concentration was （3.16±0.29）×109/ml .Those indices were not changed significantly after sterilization with irradiance of 60Co γ Ray . The maximum ratio and amount of loading gene of microbubbles was 22％ and 0.45ug/ml respectively.The appearance and diameter of microbubbles did not changed obviously after gene loading. Contrast imaging showed that the self—made lipid microbubbles could enhance the intensity of gray—scale（GS）.
Conclusions：The self—made lipid microbubbles are consistent to standard of ideal ultrasound contrast agent，which may become a new ultrasound contrast agent and a vehicle for encapsulating with genes or drugs.
Key words： Ultrasound contrast agent，60CoγRay，Gene.
